Book Image

Image Processing with ImageJ

Book Image

Image Processing with ImageJ

Overview of this book

Digital image processing is an increasingly important field across a vast array of scientific disciplines. ImageJ's long history and ever-growing user base makes it a perfect candidate for solving daily tasks involving all kinds of image analysis processes. Image Processing with ImageJ is a practical book that will guide you from the most basic analysis techniques to the fine details of implementing new functionalities through the ImageJ plugin system, all of it through the use of examples and practical cases. ImageJ is an excellent public domain imaging analysis platform that can be very easily used for almost all your image processing needs. Image Processing with ImageJ will start by showing you how to open a number of different images, become familiar with the different options, and perform simple analysis operations using the provided image samples. You will also learn how to make modifications through ImageJ filters and how to make local measurements using the selections system. You will also find the instructions necessary to record all the steps you perform so they can be saved and re-run on the same image to ensure analysis reproducibility. Finally, you will get to know some different ImageJ plugins and will learn how to implement your own.
Table of Contents (13 chapters)
Image Processing with ImageJ
Credits
About the Authors
About the Reviewers
www.PacktPub.com
Preface
Index

Selecting regions of your image


This section will deal with the process of selecting different regions in your image (lines or whole areas) so that you can perform local measurements using only those selections. Selections, or regions of interest (ROIs), are the basis of region analysis in ImageJ, and open the door to advance measuring of the information in your image, so let's devote some paragraphs to clarify the initial concepts. Let's start by drawing a few lines.

Basic selections – lines, length, and profiles

Open the tuberculosis.tif test image. It is a multichannel image (one 2D image for Red, another for Green, and the last for Blue) and we will use it because working with selections on images of more than two dimensions offers some extra possibilities. The tool to draw a line is the fifth one from the right on the ImageJ toolbar with a line icon. When you move the mouse over it, the status bar will indicate the kind of things you can do. You will probably think that there are too...