Book Image

Image Processing with ImageJ

Book Image

Image Processing with ImageJ

Overview of this book

Digital image processing is an increasingly important field across a vast array of scientific disciplines. ImageJ's long history and ever-growing user base makes it a perfect candidate for solving daily tasks involving all kinds of image analysis processes. Image Processing with ImageJ is a practical book that will guide you from the most basic analysis techniques to the fine details of implementing new functionalities through the ImageJ plugin system, all of it through the use of examples and practical cases. ImageJ is an excellent public domain imaging analysis platform that can be very easily used for almost all your image processing needs. Image Processing with ImageJ will start by showing you how to open a number of different images, become familiar with the different options, and perform simple analysis operations using the provided image samples. You will also learn how to make modifications through ImageJ filters and how to make local measurements using the selections system. You will also find the instructions necessary to record all the steps you perform so they can be saved and re-run on the same image to ensure analysis reproducibility. Finally, you will get to know some different ImageJ plugins and will learn how to implement your own.
Table of Contents (13 chapters)
Image Processing with ImageJ
Credits
About the Authors
About the Reviewers
www.PacktPub.com
Preface
Index

Modifying a recorded macro


You can open an existing macro file by navigating to Plugins | Macros | Edit... You can then do all the modifications you need (for instance, you can restart the recorder and add more commands if you need them, or comment what you did previously), and save the file again.

Note that the macro commands store the parameters used for each tool. Suppose you want to change some of them (for example, setting a rolling ball radius in the background subtraction tool of 40 instead of 30). In that case, you only need to edit the macro file and substitute:

run("Subtract Background...", "rolling=30");

By the following code:

run("Subtract Background...", "rolling=40");

Then you can rerun the macro and see the difference in the results.