Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Selecting and classifying variants with VariantAnnotation

In pipelines where we've called variants, we'll often want to do subsequent analysis steps that need further filtering or classification based on features of the individual variants, such as the depth of coverage in the alternative allele. This is best done from a VCF file, and a common protocol is to save a VCF of all variants from the actual calling step and then experiment with filtering that. In this section, we'll look at taking an input VCF and filtering it to retain variants in which the alternative allele is the major allele in the sample.

Getting ready

We'll need a tabix index VCF file; I provide one in the datasets/ch2/sample.vcf.gz file...