Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Representing raw MS data visually

The raw data of proteomics analysis is the spectra that's generated by the mass spectrometers. Each type of mass spectrometer has a different native file format in which the spectra are encoded. Examining and analyzing the spectra begins with loading in the files and coercing them into a common object type. In this recipe, we'll look at how to load the varied file types, look at the metadata, and plot the spectra themselves.

Getting ready

For this recipe, we'll need the Bioconductor package, mzR, and some files from this book's data repository, in the datasets/ch6 folder. We'll use three different files, selected not so much for the data in them, but because they each...