Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Quantifying differences between trees with treespace

Comparing trees to differentiate or group them can help researchers to see patterns of evolution. Multiple trees of a single gene tracked across species or strains can reveal differences in how that gene is changing across species. At the core of these approaches are metrics of distances between trees. In this recipe, we'll calculate one such metric to find pairwise differences between 20 different gene trees in 15 different species—hence, 15 different tips with identical names in each tree. Such similarity in trees is usually needed to compare and get distances, and we can't do an analysis like this unless these conditions are met.

Getting ready

For this...