Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Reading amplicon data from raw reads with dada2

A long-standing technique in metagenomics, particularly for those interested in bacterial microbiome studies, uses the sequencing of cloned copies (amplicons) of the 16S or 18S rRNA genes to create species profiles. These approaches can take advantage of lower throughput sequencing and the knowledge of the target sequence to classify each cloned sequence, simplifying the tricky task of assigning taxa to reads. In this recipe, we'll make use of the dada2 package to run an amplicon analysis from raw fastq sequence reads. We'll perform quality control and OTU assignment steps and use an interesting machine learning method to classify sequences.

Getting ready

For this recipe...