Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Visualizing taxonomic abundances with heat trees in metacoder

However we arrive at estimates of taxonomic abundance, it is usually helpful to create a visualization that summarizes the broad trends in the data in a single figure. One expressive and easy to interpret visualization is a heat tree. These are renderings of phylogenetic trees of the taxons of interest with data mapped onto visual elements of the render. For example, the number of times a taxon is seen may be expressed by changing the color or thickness of a tree branch. Different datasets can be easily compared by examining trees of each for differences. In this recipe, we'll construct a heat tree and customize it.

Getting ready

We need the input .biom file...