Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Splitting sequence files into OTUs

Perhaps the most common task with cleaned trimmed reads for a metagenomic shotgun experiment is to divide the sequences into OTUs. This can be achieved in many ways; in this recipe, we'll look at a method that splits sequences into subsequences of a given length and performs a type of hierarchical clustering on them to create groups.

Getting ready

The key package here is the kmer package and we'll use one of the sample fastq sequence files in the datasets/ch5/fq folder. We'll also make use of the dplyr and magrittr packages for convenience.

How to do it...

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