Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Visualizing the quality control of read-to-reference alignments

Once the alignment of reads has been performed, it is usually wise to check the quality of the alignment and ensure that there is nothing unexpected about the pattern of reads and things such as expected insert distances. This can be especially useful in draft reference genomes where unusual alignments of high-throughput reads can reveal misassemblies of the reference or other structural rearrangements. In this recipe, we'll use a package called ramwas, which has some easily accessed plots we can create to assess alignment.

Getting ready...

For this recipe, we'll need the prepared bam_list.txt and sample_list.txt information files in the datasets/ch8...