Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Correcting p-values to account for multiple hypotheses

In bioinformatics, particularly in genomics projects, we often perform statistical tests thousands of times in an analysis. But this can be a source of significant error in our results. Consider a gene expression experiment that has small numbers of measurements per treatment (often only three) but has tens of thousands of genes. A user doing a statistical test at p <= 0.05 will reject the null hypothesis incorrectly five percent of the time. Correcting for performing multiple hypotheses allows us to reduce the error rate from such analyses. We will look at a simple-to-apply method for making such a correction.

Getting ready

All of the functions we need are base R and...