Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

3D structure protein alignment with bio3d

Three-dimensional structural alignments between two molecular models can reveal structural properties that are common or unique to either of the proteins. These can suggest evolutionary or functional commonalities. In this recipe, we'll look at how to get an alignment of two protein sequences in three dimensions and view them in 3D-rendering software.

Getting ready

For this section, we need at least two external pieces of software—PyMOL and MUSCLE—a 3D structure-rendering program and a sequence aligner.

MUSCLE can be installed with conda as follows:

conda install -c bioconda muscle 

A version of MUSCLE is installed with the msa package, and bio3d can be referred...