Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Visualizing distributions of peptide hit counts to find thresholds

Every MS experiment will need some idea of the peptide hit counts that represent noise or unusual features, such as over-represented peptides in the proteome. In this recipe, we'll use some neat visualization tricks using tidyverse tools such as dplyr and ggplot to create graphics that will help you get an idea of the spread and limits of the peptide hits in your mass spectrometry experiment.

Getting ready

For this recipe, you'll require the MSnId, data.table, dplyr, and ggplot packages. We'll use the mzid file, HeLa_180123_m43_r2_CAM.mzid.gz, from the datasets/ch6 folder of this book's repository.

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