Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Identifying genomic loci that match peptides

Finding the exact places on a genome that a peptide matches to can be a challenging task, especially if the genome is one that is not represented by the original search file. In this recipe, we'll look at mixing in a classic command-line BLAST recipe to find short, nearly precise matches for peptides on a translated genome sequence to various R genomics pipelines by targeting a GRanges object of the BLAST hits.

Getting ready

For this recipe, we'll use the MSnID, dplyr, withR, GenomicRanges, and Biostrings packages and a search engine output file of Escherichia coli-derived spectra, which can be found in the PXD006247.mzXML.mzid file in this book's datasets/ch6 folder...