Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Matching spectra to peptides for verification with protViz

Although most spectra/peptide matching is done in high throughput search engines, there are times when you'd like to check the quality of competing ambiguous matches against one another, or against a completely arbitrary sequence of interest. Running the whole search engine pipeline is probably overkill, so, in this recipe, we'll look at a convenient method to run a single spectrum against a single peptide sequence and get a plot of congruence between theoretical ion sizes and those present in the spectrum.

Getting ready

For this recipe, all we need is the protViz package, the mzR package, and the MM8.mzml file from the datasets/ch6 folder of this book&apos...