Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Working programmatically with Bioconductor classes

The wide scope of Bioconductor means that there are a great number of classes and methods for accomplishing pretty much any bioinformatics workflow that you'd want to. Sometimes, though, it would be helpful to have an extra data slot or some other tweak to the tools that would help to simplify our lives. In this recipe, we're going to look at how to extend an existing class to include some extra information that is specific to our particular data. We'll look at extending the SummarizedExperiment class to add hypothetical barcode information—a type of metadata indicating some nucleotide tags that identify the sample that is included in the sequence read.

Getting ready

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