Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Applying quality control filters to spectra

Quality control of raw proteomics data is an essential step in ensuring that pipelines and analyses give believable and useful results. A large number of metrics and plots of data are needed to get a view of whether a particular experiment has been a success, and that means carrying out a lot of analysis before we start to actually derive any new knowledge from the data. In this recipe, we'll look at an integrated pipeline that carries out a wide range of relevant and useful QC steps and presents the result as a single helpful and readable report.

Getting ready

In this recipe, we'll be examining an Escherichia coli cell membrane proteomics experiment. This will require...