Book Image

R Bioinformatics Cookbook

By : Dan MacLean
Book Image

R Bioinformatics Cookbook

By: Dan MacLean

Overview of this book

Handling biological data effectively requires an in-depth knowledge of machine learning techniques and computational skills, along with an understanding of how to use tools such as edgeR and DESeq. With the R Bioinformatics Cookbook, you’ll explore all this and more, tackling common and not-so-common challenges in the bioinformatics domain using real-world examples. This book will use a recipe-based approach to show you how to perform practical research and analysis in computational biology with R. You will learn how to effectively analyze your data with the latest tools in Bioconductor, ggplot, and tidyverse. The book will guide you through the essential tools in Bioconductor to help you understand and carry out protocols in RNAseq, phylogenetics, genomics, and sequence analysis. As you progress, you will get up to speed with how machine learning techniques can be used in the bioinformatics domain. You will gradually develop key computational skills such as creating reusable workflows in R Markdown and packages for code reuse. By the end of this book, you’ll have gained a solid understanding of the most important and widely used techniques in bioinformatic analysis and the tools you need to work with real biological data.
Table of Contents (13 chapters)

Performing quality control and filtering on high-throughput sequence reads

When we have a new set of sequence reads to work with, whether that be from a new experiment or a database, we need to perform a quality control step that will remove any sequence adapters, remove reads with a poor sequence, or trim down a poor sequence, as appropriate. In this recipe, we'll look at doing that within R using the Bioconductor ShortRead package.

Getting ready

You'll need the ShortRead package and you'll need to run the code for the Finding experiments and reads from SRA/ENA recipe in this chapter. Two files are created in the last step of that recipe and we'll use one of those. Once that code is run, the file should...